With life sciences becoming a dominant urban industry, many cities and developments are wrestling with integrating these specialized buildings into thriving urban, mixed-use communities. Providing a synopsis of the evolution of the 43-acre Cambridge Crossing through three cycles of ownership changes and 20 years of development, the panel will offer an informative case study with insights on how to meet changing market needs, transportation demands, and community attitudes while preserving the integrity of a large-scale master plan. The discussion will address five critical issues through the lenses of master planner, developer, and community leader: stewardship of a large-scale mixed-use project over a 20-year time frame, designing for distinction within an existing urban framework and ensuring high-quality architecture and establishment of a large-scale ecological transportation system, the role of the public sector in ensuring the flexible evolution and quality of private development, planning and developing a life-science innovation cluster, and the role of programming and retail initiatives by the developer to make an inviting and inclusive public realm.